Facscan and facscalibur cleaning and shutdown instructions. Database contains 3 bd facscanto ii manuals available for free online viewing or downloading in pdf. We also provide consulting services on multicolor panel design and flow cytometry data analysis. Facscan cleaning and maintenance purdue university. These instructions need to be carried out after the completion of your experiment. Although these symbols appear in color on the instrument, they are in black and white throughout this users. Technical manuals and documentation bd lsr ii octagon and trigon filter configurations most recent, 52005 bd lsr ii overview from bd biosciences, 92002 bd lsr ii user guide from bd. Some of the illustrations shown in this manual may contain accessories and optional equipment that are not included on your system. Soluble helminth products suppress clinical signs in. Does not support bd 4color upgrade and sorting options on facscalibur or facsort cytometers installing the flowjo collectors edition fjce. Bd serves and supports our customers with full offerings beyond our individual products, enabling integrated solutions across healthcare systems to comprehensively improve care and lower costs. Subsequently a tube filled with destilled water was run over 1015 minutes same settings as sodium hypochlorite solution.
Bd facscalibur, bd facscan, bd facsort, or bd lsr flow cytometer. With up to four different laser configurations and 14 parameters, the cytometer is fully optimized to work with bd horizon brilliant dyes, so you can focus on what matters mostyour research. Intended use becton dickinson simultest imklymphocyte is a twocolor direct immunofluorescence reagent kit for enumerating percentages of the following mature human leucocyte subsets in erythrocytelysed whole blood lwb. Rising costs, decreasing reimbursement, labor intensive manual procedures, fewer resources, and higher demands for efficiencythey are all challenges todays clinical laboratory faces. We are bostonlocated flow cytometry core facility equipped with analyzers 5laser bd lsr ii sorp, a facscanto and a facscalibur and cell sorters 4 laser16 color facs aria ii sorp and a 5 laser25 color moflo astrios. This document is designed to help the newcomer download, install and navigate from freescan, which is designed to work with the uniden dma scanners. Find the user manual and the help you need for the products you own at manualsonline. We continue to innovate the most sensitive detection technology for researchers, utilizing side scatter technology for nanoparticle detection to. View online user manual manual for bd facscanto ii measuring instruments or simply click download button to examine the bd facscanto ii guidelines offline on your desktop or laptop computer.
The bd cba is not recommended for use on streaminair instruments where signal intensities may be reduced, adversely effecting assay sensitivity. It analyzes cells as they pass through a focused laser beam one at a time in a moving fluid stream. Other procedures can be found in the bd facsort users. The bd facscelesta multicolor cell analyzer helps rapidly and accurately analyze cells in a compact, affordable package. Todays bd facscanto system features a fixedalignment flow cell in the fluidics system that minimizes startup time and improves reproducibility. How a flow cytometer works thermo fisher scientific us. All rights reserved page 5 of 33 document 246410 rev to start a new exam, enter patient information in the create new exam window. This users guide contains the instructions necessary to operate and maintain. Bd compbeads compensation particle sets are designed to optimize compensation settings in multicolor flow cytometry. Chapter 1 using this manual this manual contains tool usage instructions. At bd, we seek to usher in a new era of healthcare by bringing medical products, capabilities and solutions to every corner of the world.
Technical center equipment manuals, parts lists, and diagrams. Data files and workspaces stored in my documents will not be accessible to other limited users but will be available to administrator accounts. User manuals, guides and specifications for your bd facscanto ii laboratory equipment, measuring instruments. Conventions the following conventions are used in these instructions. For information on how to operate and maintain your flow cytometer, refer to the instrument users guide. This document is for information and instruction purposes. Invitrogen alignflow flow cytometry alignment beads are reliable references for aligning, focusing, and calibrating flow cytometers. A bd facscan flow cytometer can be used for routine research applications, immunophenotyping, and. The bd facscalibur instructions for use assumes you have a working knowledge of the mac os x operating system.
It analyzes cells as they pass through a focused laser beam one at a time in a moving. Bd lsr ii users guide indiana university bloomington. Find flow cytometer equipment for sale, or wanted from the worlds largest medical equipment marketplace. Autocrit centrifuge and adams microhematocrit ii centrifuge. The bd facscan flow cytometer becton dickinson was calibrated with calibrate 3 color beads becton dickinson, using facscomp 4. Learn about bd life sciences diagnostic systems qcpi manuals. Instruments available for use at the flow cytometry core facility, institute of cell and molecular science, barts and the london, queen mary, university of london. Bd facscalibur instructions for use bd biosciences. Hts customer care kits 52 pages laboratory equipment bd accuri c6 instrument manual. Labexchange is offering you the transfer of instructions in pdf format of numerous laboratory and analysis devices free of charge. Laboratory equipment bd facscan instructions 2 pages. Bd biosciences recommends that firsttime users of this cytometer take advantage of operator training offered with the sale of every new cytometer. The latest version of fjce can be found on the cytek development website in the products flowjo collectors edition section. Remove black metal plate on top of sheath fluid tank push forward, then lift off.
The three main components of a flow cytometer are the fluidics, optics, and electronics figure 1 the fluidics system of a flow cytometer is responsible for transporting sample from the sample tube to the flow cell. Find flow cytometer listings for sale or wanted on. Flow cytometer calibration and size reference beads. To change your password as a limited user, go to user accounts in the control panel, and follow the instructions to change passwords.
Bd facs clean solution is a bleachbased cleaning agent for daily use in cytometer maintenance. Bd facsaria ii, bd facsaria iiu, bd facsaria iii, bd facsaria iiiu, special order research product sorp bd. The bd facscanto flow cytometer reference manual assumes you have a working knowledge of basic microsoft windows operation. You assume total responsibility and risk associated with using this manual. Bd, bd logo and all other trademarks are property of becton, dickinson and company. Baclight bacterial stains b35000 baclight green bacterial stain b35001 baclight red bacterial stain figure 1. This manual has be produced only as a guide to using freescan and helping in diagnosing.
When you are done with your experiment, place a facs tube with 2 mls 10% bleach on the sip tube and run for 5 minutes on hi. Laboratory equipment bd facsort user manual 14 pages laboratory equipment bd facsjazz user manual. Bectondickinson facscalibur flow cytometer instructions. Streaminair instruments include the bd facstar plus and bd facsvantage bd biosciences immunocytometry systems, san jose, ca flow cytometers. For information on how to operate and maintain your flow cytometer, see your cytometer manual. Becton dickinson facscan bench top automated flow cytometer. Oct 25, 2016 bd lsr ii flow cytometer quick user guide v. Bd becton, dickinson and company diagnostic systems. The bd facsdiva software reference manual includes instructions or descriptions for installation and setup, workspace components, acquisition. We have facsdiva, cellquest pro and flowjo analysis stations. Refer to the bd facscan system users guide for troubleshooting the facscan instrument. Once through the flow cell and past the laser, the sample is either sorted in the case of cell sorters or transported to waste. Bd facscanto flow cytometer reference manualk pdf4pro.
In 1985, cell analyzers allowed up to three color analysis, such as epics c analyzer leif et al. This procedure is mandatory for maintaining proper instrument operation. A bd facscan system the facscan system is an automated. Bd facscanto ii manuals and user guides, laboratory. Page 1 bd high throughput sampler users guide for the bd lsr ii bd facscanto bd facscanto. Operation of the 16bit version of modsim is similar, but is not detailed in this document. Technical center equipment manuals, parts lists, and diagrams equipment and supplies. Because many cytometer functions are controlled by bd facsdiva software, this guide also contains information about software features required for basic cytometer setup and operation.
Bd lsr ii users guide describes procedures necessary to operate and maintain your bd lsr ii flow cytometer. Page 12 sample oring replacement the following section describes the monthly cleaning procedure. Sony sy3200 haps 1 and 2 bd facscan system bd cellquest guide core facscan guide. These instructions describe how to operate your bd facscalibur flow cytometer using bd facstation software. Becton dickinson facscan the bd facscanto system breaks new frontiers in flow cytometry by providing an efficient, easytouse yet powerful sixcolor benchtop analyzer. Becton dickinson pty ltd, 4 research park drive, macquarie university research park, north ryde nsw 21, australia becton dickinson limited, 8 pacific rise, mt. This recommendation was exactly the one i found in the german version of facscan user s manual by bd. Notice in bd cellquest pro software, choose cytometer status. Analyzes individual cells as they pass in a moving stream through a laser beam. Flow cytometer 31 pages summary of contents for bd facscanto. National laser offers the widest variety of replacement lasers for life science inspection equipment. Bs lsrfortessa cell analyzer users guide bd biosciences. Flow cytometer 204 pages laboratory equipment bd 644787 user manual. In addition, you need the security module provided with cellquest.
Since then, there have been many innovations in the product line, reflecting our commitment to reach to ever higher standards. These fluorescently stained polystyrene microspheres are highly uniform with respect to size and fluorescence intensity figure 3, and are designed to approximately replicate the size, emission wavelength, and intensity of biological samples. Bd facscanto ii measuring instruments user manual manual. After passing calibration, the samples were processed and 10,000 events collected. Bd facscanto ii measuring instruments user manual manual pdf. Microbial enumeration in 4 minutes pass fail results for presence absence of microbial contamination in 24 to 48 hours yeast, bacteria and mold detection in a single run. Flow cytometry histograms from a bd facscan bd biosciences, san jose, ca showing fluorescence of live and dead grampositive and gramnegative bacteria stained with the baclight bacterial stains. Soluble products of helminths differentially suppress dendritic cell cytokine production. Facscalibur system users guide v preface facscalibur is the becton dickinson immunocytometry systems bdis modular benchtop flow cytometer designed for applications ranging from routine clinical to advanced research. Unless otherwise noted, bd, the bd logo and all other trademarks are property of becton, dickinson and company. National laser replacement lasers for life science. Lotus esprit world cannot be held responsible for anything you do with this software, cable and manual.
Providing excellence in accuracy, reliability and reproducibility. Competition started between the different flow cytometer manufacturers concerning the simultaneous analysis capacity of fluorescence. Laboratory equipment bd facscan instructions 2 pages laboratory equipment bd lsr ii user manual. Reading this material and answering the questions at the end of each. Page 1 bd high throughput sampler user s guide for the bd lsr ii bd facscanto bd facscanto. User bulletin directions for the use of the sheathethanol filter 122018 overview these directions are for the use of the sheathethanol filter, part number 661744, for the following instruments. Contact your sales repres entative for availability of accessories and optional equipment. It uses fixedalignment lasers that transmit light through a flow cell to configurable octagon and trigon detector arrays. About this manual this manual describes how to use bd facsdiva software. Wellington, auckland, new zealand ec rep bd facsdiva software reference manual for version 8. Flow cytometers, reagents, software beckman coulter. Directions for the use of the sheathethanol filter bd. Bd, the bd logo, bd calibrite, bd facs, bd facscalibur, bd facscanto, bd. Bd facscanto ii manuals and user guides, laboratory equipment.
Flow cytometers, cell sorters and cell preparation systems. We offer specialized instruments for routine or high complexity flow cytometric analysis designed with a deep understanding of the clinical workflow and the needs of the hematopathologist. Bd life sciences diagnostic systems qcpi manuals bd. Read the section of the workbook or users guide specific to the operation you are. Bd facscan anaylser at flow cytometry core facility.
The bd facsdiva software reference manual assumes that you have a working knowledge of basic microsoft windows operation. Although these symbols appear in color on the instrument, they are in black and white throughout this user s. Mentor graphics reserves the right to make changes in specifications and other information contained in this publication without prior notice, and the. Other procedures can be found in the bd facsort user s. Modsim following is a concise users manual for the operation of modsim32. The first bd facscanto system was introduced in 2004. Baclight bacterial stains thermo fisher scientific. It incorporates many technology advances first developed in the bd lsr ii expandable analyzer and the bd facsaria cell sorter.
427 575 732 795 748 294 1140 1470 1465 1284 206 1438 350 485 16 985 844 1299 611 930 1502 1503 224 666 1082 44 528 344 25 133 974 1142 932 899 1300 1082 573 419 742 1094 238 652 620